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. Author manuscript; available in PMC: 2023 Aug 2.
Published in final edited form as: Cell Metab. 2022 Jul 14;34(8):1151–1167.e7. doi: 10.1016/j.cmet.2022.06.010

Figure 5: Arg restriction induces a GNC2-mTORC1 mediated inhibition of protein synthesis.

Figure 5:

A, Schematic representation of GCN2-SESTRIN2-mTOR mediated regulation of protein synthesis. B, C, Protein synthesis (assessed by puromycin pulse-chase) in SNU-398 cells cultured in control or Arg free conditions treated with 1 μM GCN2iB (B), and densitometric quantification (C). D, E, Western blot (D) and mRNA expression analysis (E) of SESTRIN2 (SESN2) in HCC cells cultured in control and Arg free conditions. F, G, Protein (F) and mRNA (G) levels of SESTRIN2 (SESN2) in HCC cells cultured in control and Arg free conditions after 1 μM GCN2iB treatment. H, I, Phospho-mTOR (p-mTOR) and total mTOR levels in control and Arg free cultured HCC cells (H) and p-mTOR/mTOR-total ratios determined by densitometric analysis (I). J-L, Phospho-S6K (p-S6K), total S6K, phospho-S6 (p-S6), and total S6 in HCC cells cultured in control and Arg free conditions (J). Densitometric quantification of p-S6K/S6K ratio (K) and p-S6/S6 ratios (L). M-P, Phospho-mTOR (p-mTOR), total mTOR, phospho-S6K (p-S6K), total S6K, phosphorS6 (p-S6), and total S6 in SNU-398 cells cultured in control and Arg free conditions after 1 μM GCN2iB or DMSO treatment (M). Densitometric quantification of p-mTOR/mTOR (N), p-S6K/S6K (O), and p-S6/S6 (P). See also Figure S5.