Figure 5.
Alterations in Dcx expression levels in the cortices of NexKO embryos, neonates, and young adults compared to age-matched controls: (A) Dcx expression in the cortices of E15.5 embryos demonstrate a higher percentage of neuronal layers in the cortices of NexKO embryos compared to controls, as measured by the higher ratio of the thickness of Dcx-positive neuronal layers over the total cortical thickness. (B) Representative confocal images showing staining of cell nuclei (DAPI, in blue) and Dcx (green) in the cortices of E15.5 control and NexKO embryos. (C) Comparable Dcx mRNA levels in P1 NexKO mice compared to controls. (D) A trend towards an increase in miR-34c-5p levels in the cortices of P1 NexKO mice compared to controls. (E) Significantly decreased expression levels of Dcx mRNA in the cortices of P30 NexKO mice compared to controls. Data are shown as the mean ± SEM. * p < 0.05, *** p < 0.005, # p = 0.08 (A) two-tailed Mann–Whitney test, (C–E) two-tailed t-test with Welch’s correction. (A) n = 4 per group; (C) n = 12 control; n = 11 NexKO; (D) n = 11 per group; (E) n = 9 per group. ns: non-significant.