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. 2022 Jan 4;11(1):158. doi: 10.3390/cells11010158

Figure 4.

Figure 4

miR-34c-5p does not directly regulate RHEBL1 mRNA: (A) Significantly increased expression levels of Rheb1 mRNA in the cortices of P30 NexKO mice compared to controls. (B) Reduced levels of RHEBL1 mRNA 72 h post-transfection of hsa-pre-miR-34c in SH-SY5Y cells, compared to controls. (C) Sequences of the mature hsa-miR-34c-5p and the Renilla/firefly luciferase psiCHECK2 constructs (WT and MUT) under the regulation of RHEBL1 3’UTR, around the hsa-miR-34 binding site; miR-mRNA binding sites are shown in bold; mutated nucleotides are in red. (D) Reduced luciferase activity levels of the mutated RHEBL1 3’UTR luciferase construct, 72 h post-transfection of hsa-pre-miR-34c in HEK-293T cells, compared to the activity levels of the WT luciferase construct. Data are shown as the mean ± SEM. * p < 0.05, # p = 0.08; (A,B) two-tailed t-test (B) with Welch’s correction; (D) one-tailed t-test. (A) n = 10 control; n = 9 NexKO; (B) n = 6 per group; (D) n = 4 per group. OE: overexpression.