Figure 2.
Whole-cortex small RNA sequencing analysis of P30 NexKO mice. Small RNA sequencing analysis of total RNA extracted from the cortices of P30 NexKO mice compared to controls revealed altered miRNA expression. (A) Volcano plot representation of differentially expressed miRNAs. Data are shown as the log2 fold change in counts in NexKO mice compared to controls. (B–H) Seven downregulated and (I) one upregulated miRNA in the cortices of NexKO mice compared to controls. Data are shown as medians ± quartiles. n = 3 control; n = 2 NexKO. (J) Gene Ontology analysis of verified targets of the murine miR-34 cluster, presenting biological pathways significantly associated with verified targets of the miR-34 cluster; among them are pathways associated with axonogenesis, forebrain development, gliogenesis, and developmental growth.