TABLE 2.
Phenotypes of ada mutants
| Double mutanta | Phenotypee |
|---|---|
| ada1Δ swi1Δb,d | Dead |
| ada1Δ snf5Δ2c | Dead |
| ada1Δ srb5Δc | Dead |
| ada1Δ gal11Δb,d | Dead |
| ada2Δ swi1Δ | Alive, sick |
| ada2Δ snf2Δd | Alive, sick |
| ada2Δ srb5Δ | Alive, slow growing |
| ada2Δ srb2Δ | Alive, slow growing |
| ada2Δ gal11Δ | Alive |
| ada3Δ swi1Δd | Alive, sick |
| ada3Δ snf2Δ | Alive, sick |
| ada3Δ srb5Δ | Alive, slow growing |
| ada3Δ srb2Δ | Alive, slow growing |
| ada3Δ gal11Δd | Alive |
| ada3Δ sin4Δ | Alive |
In each cross, genetic markers allowed us to unambiguously identify all relevant markers in every tetrad. The parents for the crosses in the order listed are as follows: FY602 × FY1254, FY1658 × FY1557, FY1557 × L937, FY602 × FY1657, FY1548 × FY1254, FY602 × FY1656, FY1548 × L937, FY1548 × FY1359, FY1554 × FY1657, FY602 × FY1254, FY1542 × FY1656, FY1542 × L937, FY1542 × FY1359, FY602 × FY1657, and FY1253 × FY1545.
Viability was scored by the ability of the double mutant to lose a URA3-marked wild-type SWI1 (BD1) or GAL11 (FB565) plasmid as determined by growth on 5-FOA medium (alive, growth on 5-FOA; dead, no growth on 5-FOA).
Viability was determined by failure to recover any double mutants after dissecting 20 tetrads.
For all crosses in which FY602 is listed as the MATa parent, the double mutant was constructed by first creating a diploid between FY602 and the corresponding MATα parent. This diploid was then transformed with a PCR fragment containing the relevant ADA ORF completely disrupted by the HIS3 gene. Those single purified His+ diploid transformants that displayed correct ADA knockout integration (as described in Materials and Methods) were used in tetrad analysis.
Sick, extremely small colonies after 2 to 3 days of growth at 30°C. Slow growing, colonies were moderately smaller than those of wild-type strains after 2 to 3 days of growth at 30°C.