FIGURE 4.
Tukey's box and whisker plots illustrate the changes in ribosome biogenesis regulatory genes CMYC (A), EIF4E (B), NIP7 (C), TIF1A (D), POLR1B (E), and UBF (F) after consumption of mMPC, CAS, or MPC protein beverages at 2 and 4 h in healthy young men. Middle horizontal line represents the median, values displayed as 95% CI, n = 10/group. Data were analyzed with 2-factor repeated measures ANOVA. *Indicates significant difference to baseline ( P ≤ 0.05). #Indicates when 4 h is significantly different to 2 h (P ≤ 0.05). A horizontal bar indicates a main effect of time for all 3 groups. CAS, calcium caseinate; EIF4E, eukaryote initiation factor 4E; mMPC, mineral modified milk protein concentrate; MPC, milk protein concentrate; NIP7, 60S ribosome subunit biogenesis protein NIP7 homolog; POLR1B, RNA polymerase I subunit B; TIF1A, transcription initiation factor IA; UBF, upstream binding factor; 3HK, the 3 most stable housekeeper genes.