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. Author manuscript; available in PMC: 2019 Oct 7.
Published in final edited form as: Nat Immunol. 2019 Jul 1;20(8):1023–1034. doi: 10.1038/s41590-019-0421-2

Fig. 5 |. Visualization of TREM1 induction using PET imaging demonstrates activation of peripheral myeloid cells in spleen and intestine after MCAo.

Fig. 5 |

a, Three-dimensional sagittal maximum intensity projection PET/CT images of representative sham and MCAo mice, 36 h after MCAo, injected with either [64Cu]TREM1-mAb or [64Cu]sotype-control-mAb at 12 h after MCAo. b, Quantification of in vivo PET signal in brain 36 h after MCAo (n = 9 biologically independent samples per group, mean ± s.e.m.; Student’s two-tailed t-test, *P < 0.05). c, Quantification of in vivo PET signal in spleen (n = 9 biologically independent samples per group, mean ± s.e.m. except for sham, where n = 3; Student’s two-tailed unpaired t-test, *P < 0.05; ***P < 0.001). d, Quantification of in vivo PET signal in intestine (n = 9 biologically independent samples per group, mean ± s.e.m. except for sham, where n = 3; Student’s two-tailed t-test, *P < 0.05; ***P < 0.001). e, Representative autoradiography images of coronal brain sections, cresyl violet staining and overlay of autoradiography and cresyl staining from mice imaged with [64Cu]TREM1-mAb or [64Cu]isotype-control-mAb 36 h after MCAo. f, Quantification of ex vivo brain autoradiography (AR) (n = 9–10 biologically independent samples per group, mean ± s.e.m.; twotailed Student’s unpaired t-test, *P < 0.05).