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. 2019 Mar 27;8:e44210. doi: 10.7554/eLife.44210

Figure 4. Methyltransferase expression in T cells.

Figure 4.

Abundance of (a) CMTR1 and RNMT cap methyltransferases (b) METTL3 m6A RNA methyltransferase and (c) NSUN2 m5C methyltransferase expression in naïve, TCR activated and effector Th1 CD4+ T cells (plotted as in 3 c). (e) DNA methyltransferases use SAM as a methyl donor to methylate CG residues. The concentration of the DNA methyltransferase complexes expressed in naïve CD4+ T cells (naïve), 24 hr TCR- stimulated (aCD3/aCD28, IL2/12) CD4+ T cells (TCR) and in vitro generated Th1 cells (Th1). (f) The polycomb repressor complex 2 (PRC2) uses SAM to methylate lysine residues for example K27 on histone tails. Concentration of the PRC2 components calculated from proteomics data from naïve CD4+ T cells (naïve), 24 hr TCR- stimulated CD4+ T cells (TCR) and in vitro generated Th1 cells (Th1). (g) Flow cytometry plots showing EZH2 staining in naïve CD4+ T cells, CD4+ T cells stimulated through the TCR (aCD3/aCD28, IL2/12) for 18 hr and in vitro generated Th1 cells. MFI are shown on the graph. (h) Concentrations of histone methyltransferases calculated from proteomics data of naïve CD4+ T cells (naïve), 24 hr TCR- stimulated CD4+ T cells (TCR) and in vitro generated Th1 cells (Th1). Histone methyl modifications are indicated. (Error bars are mean ± s.d. Data are from three biological replicates.).