Figure 4.
Tofacitinib restores down‐regulation of ABCA1 protein expression induced by IFNγ in THP‐1 cells pretreated with HFD rabbit serum or h‐oxLDL. Western blot of ABCA1, ABCG1, LXRα and phospho‐STAT1 in THP‐1 foam cells obtained after incubation with 4% HFD rabbit serum (A, left panel, n = 6) or 50 μg·mL−1 h‐oxLDL (B, right panel, n = 7) for 24 h. Cells were exposed to IFNγ (50 ng·mL−1) and/or tofacitinib (0.5 or 2 μM) for an additional 24 h. Results are normalized to those of tubulin and expressed as fold change of the HFD serum‐only or oxLDL‐only group. Bars show the mean and SEM. Mann–Whitney test; a: P < 0.05 versus basal media, b: P < 0.05 versus HFD serum (A) or oxLDL (B), c: P < 0.05 versus HFD serum + IFNγ (A) or oxLDL + IFNγ (B); d: P < 0.05 versus HFD serum + IFNγ + 0.5 μM TOFA (A) or oxLDL + IFNγ + 0.5 μM TOFA (B).