Figure 6.
TNFα-mediated repression overcomes activation of EAAT2 by NF-κB. (A) The effect of TNFα on EGF-, IKKβ- or p65-mediated activation of the wild-type EAAT2 reporter was tested. Normalized RLUs represent luciferase units normalized to Renilla control reporter activity. (B) The ability of IKKβ to regulate the triple mutant EAAT2 reporter was tested and compared to that of wild type. (C) TNFα-mediated repression is specific to the EAAT2 promoter. The activity of a 3x-κB-luciferase promoter containing consensus NF-κB sites from the MHC class I promoter was tested in the presence of TNFα or EGF.