FIG 2.
Expression of BART miRNAs in the cells infected with the recombinant viruses. (A) The expression levels of 13 BART miRNAs (four from cluster 1 and eight from cluster 2) in the B95.8v-infected and the BART(+)v-infected HEK293 cells, AdAH cells, and LCLs were examined by Northern blotting. Total RNA extracted from the NPC-derived C666-1 cell line was used as a control. The expression of U6 snRNA was used as a loading control. (B) TaqMan small RNA assay was used to determine relative expression levels of BART17-5p and BART22 in the BART(+)v-infected HEK293 cells, AdAH cells, and LCLs. The values of miRNA expression levels were normalized by those of hsa-miR-16 expression levels among different cells. The average miRNA expression levels in the LCLs were adjusted to 1.