FIGURE 9.
Rapamycin and NALA affect the glycolytic capacity and reserve of activated T cells. WT cells were isolated as described in the legend to Fig. 3, activated with anti-CD3/anti-CD28 (co-stimulation), and incubated subsequently with 100 nm rapamycin or 20 mm NALA for 24 h. A and B, T cell metabolic profile was determined by a Seahorse XF24 analyzer measuring ECAR as described in the legend to Fig. 8. C, P-S6, S6, P-4EBP-1, and 4EBP-1 proteins were determined by Western blotting. β-Tubulin was used as a loading control. All values are the mean ± S.D. of a single representative experiment repeated three times; *, p ≤ 0.05 as compared with untreated cells.