Table 1.
Body weights, fat mass, leptin, and inflammation marker levels
| DIO diet |
CON diet |
Diet | Treatment | Diet × Treatment |
|||
|---|---|---|---|---|---|---|---|
| AL (n = 7) | WR (n = 8) | AL (n = 8) | WR (n = 8) | ||||
| Dataa | |||||||
| Body weight (g) | 52.0 ± 1.0c | 33.6 ± 1.0d | 32.2 ± 1.1d | 22.0 ± 0.6e | F = 292.5** | F = 243.9** | F = 20.2** |
| Fat-free mass (FFM, g) | 23.5 ± 0.5c | 20.0 ± 0.3d | 20.3 ± 0.3d | 15.1 ± 0.4e | F = 111.4** | F = 135.4** | F = 5.4* |
| Fat mass (FM, g) | 20.1 ± 1.0c | 7.0 ± 0.6d | 5.1 ± 0.7d | 1.8 ± 0.1e | F = 230.6** | F = 151.8** | F = 53.3** |
| Leptin (ng/ml) | 121.7 ± 14.9c | 25.1 ± 2.9d,* | 14.0 ± 3.6d | 9.0 ± 1.3d | F = 75.8** | F = 51.0** | F = 41.4** |
| mRNA levelsb | |||||||
| F4/80 | 1.06 ± 0.06c | 1.06 ± 0.09c | 1.0 ± 0.08c | 0.58 ± 0.05d | F = 14.4** | F = 8.9** | F = 9.2** |
| Slc25a25 | 1.08 ± 0.06c | 1.01 ± 0.06c | 0.63 ± 0.06d | 0.60 ± 0.04d | F = 40.9** | F = 0.6 | F = 0.1 |
| DioII | 1.34 ± 0.46c | 0.06 ± 0.03d | 0.87 ± 0.26c,d | 1.64 ± 0.19c | F = 4.1 | F = 0.9 | F = 14.3** |
| ll10 | 0.87 ± 0.14 | 1.63 ± 0.23 | 1.17 ± 0.24 | 0.99 ± 0.23 | F = 0.6 | F = 1.9 | F = 4.7* |
| Tnfα | 1.07 ± 0.23 | 1.64 ± 0.15 | 1.58 ± 0.14 | 1.36 ± 0.19 | F = 0.5 | F = 1.0 | F = 5.1* |
| iNos | 1.29 ± 0.10c | 0.79 ± 0.08d,e | 0.72 ± 0.03e | 1.06 ± 0.10c,d | F = 3.6 | F = 1.0 | F = 28.7** |
| Cd11c | 1.11 ± 0.16c | 0.86 ± 0.09d | 0.86 ± 0.08d | 0.71 ± 0.08d | F = 3.7 | F = 3.6 | F = 0.2 |
| Saa3 | 2.2 ± 0.55c | 0.89 ± 0.34d | 0.03 ± 0.02e | 0.02 ± 0.02e | F = 23.5** | F = 4.4* | F = 4.3* |
| Pai1 | 1.81 ± 0.29c | 1.25 ± 0.24c | 0.1 ± 0.05e | 0.18 ± 0.03e | F = 54.8** | F = 1.6 | F = 2.8 |
| Angptl4 | 1.80 ± 0.23c | 0.72 ± 0.08d,e | 1.07 ± 0.09d | 0.38 ± 0.12e | F = 15.6** | F = 42.6** | F = 2.1 |
Body weight, body composition, and circulating leptin concentrations (mean ± s.e.m.): previously published data (6).
mRNA levels (normalized to cyclophilin b and presented as arbitrary units) for inflammation markers measured by quantitative reverse transcriptase PCR in inguinal fat pads (mean ± s.e.m.). Data not marked by same letter are significantly different by two-way ANOVA with Tukey honestly significant difference post hoc analysis. Direct t-tests were conducted for body weight and body composition of weight-reduced diet-induced obese (DIO-WR) and ad-libitum control diet-fed mice (CON-AL) (*P < 0.05).
Significance of two-way ANOVA factors on the right hand side are denoted by *P < 0.05 and
P < 0.01.