Figure 6. 3′ UTR luciferase activity assays confirm miR-mediated interactions between key drivers of glioma.
(A) 3′ UTR luciferase activity of PTEN, PDGFRA, RB1, RUNX1 and STAT3 were measured in SNB19 cells at 24h following siRNA mediated silencing of PTEN and RB1 compared to non-targeting siRNAs (NT5) as control (in black). (B) Results in SNB19 were replicated in SNF188 cells. Similar to Figure 5, Y-axes start at 0.5. Data are represented as mean +/− SEM.