Fig. 4.
STAT5 signals profoundly augment survival of all LCMV-specific CD8 T cells during and after infection. Activated P14 Ly5.1+ CD8 T cells were transduced with CA-STAT5, DN-STAT5, or CTRL RVs that express Thy1.1. Small numbers (∼2,500) of RV+ (Thy1.1+) P14 CD8 T cells were transferred into congenic Ly5.2+ C57BL/6 mice that were infected with LCMV. (A) Contour plots showing the percent RV+ (Thy1.1+) and RV− (Thy1.1−) P14 CD8 T cells in PBMCs at days 6 and 30 p.i. (B) Line graphs show the number of CA-STAT5 (red) and CTRL RV+ (black) (Thy1.1+) P14 CD8 T cells recovered from the spleen at different times postinfection. (C) As in B, total numbers of splenic KLRG1hi IL-7Rαlo, KLRG1lo IL-7Rαhi, KLRG1hi IL-7Rαhi, and KLRG1lo IL-7Rαlo CA-STAT5 (red) and CTRL RV+ (black) (Thy1.1+) CD8 T cells after LCMV infection are shown in the line graphs. Data shown are representative of the mean (±SEM) of 5–20 animals from five separate experiments. All statistics were calculated with Student's t test. ***P < 0.0005, **P < 0.005, *P < 0.05.