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FoxO1+ cells in pancreatic ducts. Pancreatic immunohistochemistry was performed with anti-FoxO1 (A and D; red) and anti-insulin (B and E; green) antibodies in 3-month-old male WT mice of mixed C57BL × 129sv background. (A to C) All insulin-positive juxtaductal cells coexpress FoxO1 (yellow in the merged channels). (D to F) FoxO1+ Ins− cells (red) are located within ducts (D and F). Arrows indicate occasional cells within ducts that express FoxO1 but not insulin.
