Figure 3. MyD88-negative NOD mice are protected from diabetes by the gut microbiota.
- Diabetes incidence in SPF NOD.MyD88KO (J) females and control heterozygous NOD.MyD88KO/+ littermates treated with a broad-spectrum antibiotic from birth.
- Diabetes incidence in GF NOD.MyD88KO and control MyD88KO/+ mice. Incidence is shown for male mice; 100% of female NOD.MyD88KO and NOD.MyD88KO/+ female GF mice became diabetic (Supplemental Fig.1).
- Diabetes incidence in gnotobiotic male NOD.MyD88KO and control NOD.MyD88KO/+ mice colonized with a consortium of 6 bacterial strains (ASF 361,519,356,492,502, and 500; see Supplemental results for descriptions). The incidence of diabetes in gnotobiotic NOD.MyD88KO mice was significantly different from the incidence in GF NOD.MyD88KO animals (p<0.001), and in gnotobiotic NOD.MyD88KO/+ mice (p<0.05) (Kaplan Meier test).
- Histological scores of islet destruction in SPF, GF and ASF-colonized NOD.MyD88KO/+ and NOD.MyD88KO mice. Mice in all groups were males, except for the SPF NOD.MyD88KO group, which included both genders.