Table II.
Mutations in lst8 cause derepression of Rtg1/3p-dependent transcription
| Strain
|
PCIT2-LacZ expression on glutamate medium
|
Standard deviation
|
|---|---|---|
| (β-galactosidase units) | (β-galactosidase units) | |
| WT | 2.4 | 0.6 |
| lst8-1 | 9.3 | 4.1 |
| lst8-6 | 251.3 | 10.7 |
| lst8-7 | 83.9 | 8.8 |
| mks1Δ | 729.1 | 39.7 |
| ure2Δ | 4.2 | 1.5 |
| rtg2Δ | 0.8 | 0.1 |
| rtg2Δ lst8-1 | 1.4 | 0.8 |
| rtg2Δ lst8-6 | 147.8 | 13.9 |
| rtg2Δ lst8-7 | 33.7 | 2.4 |
| rtg2Δ mks1Δ | 752.4 | 80.4 |
Strains were ura3-52 (CKY772), lst8-1 ura3-52 (CKY773), lst8-6 ura3-52 (CKY774), lst8-7 ura3-52 (CKY775), mks1Δ ura3-52 (CKY776), ure2Δ ura3-52 (CKY777), rtg2Δ ura3-52 (CKY795), rtg2Δ lst8-1 ura3-52 (CKY796), rtg2Δ lst8-6 ura3-52 (CKY797), rtg2Δ lst8-7 ura3-52 (CKY798), and rtg2Δ mks1Δ ura3-52 (CKY799) containing pEC261.