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Ubiquitin ligase activity of Rsp5p is required for mitochondrial inheritance. Cells harboring the smm1 mutation (MYY823) were transformed with centromere-based plasmids encoding wild-type RSP5 (pRS316-RSP5) or rsp5 bearing the C777A mutation (pRS316-CA). (A) Transformants were incubated on YPD medium at 37°C for 48 h. (B) Cells were cultured in minimal medium lacking uracil, shifted to 37°C for 4 h, stained with DASPMI, and examined by fluorescence microscopy. Bar, 2 μm.
