Figure 4.
Primary recruitment of ERα to the E2-responsive enh2 in MCF7 cells. ChIP assays were performed to analyze ERα (A), p300 (B), and PolII (C) recruitment to the CCND1 regulatory regions upon E2 stimulation of MCF7 cells. Amounts of immunoprecipitated DNA were normalized to inputs and reported relative to the amount obtained at +8000 bp in the absence of ligand, which was set to 1 (indicated by the horizontal black dotted line). (D) ChIP of ERα ectopically expressed in MDA-MB-231 was used to verify the cell-type-specific activity of enh2. Data were expressed as fold chromatin enrichment over empty control plasmid-transfected cells. All results are means ± SE from two to four independent experiments.