Figure 3.
Recruitment of p300 and PolII to the CCND1 gene regulatory regions. ChIP assays were used to analyze p300 (solid line) and PolII (dotted line) recruitment to the CCND1 regulatory regions in HeLa (A), MCF7 (B), and J110 (C) cells. Amounts of immunoprecipitated DNA were normalized to inputs and reported relative to the amount obtained at +8000 bp, which was set to one (indicated by the horizontal black dotted line). The +8000-bp site was used to normalize all the ChIP data in this study because it represented a negative control for transcription factor and coactivator binding (only low amounts of PolII were present at this site). Results are means ± SE from two to four independent experiments.