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. 2023 Aug 28;16:3699–3714. doi: 10.2147/JIR.S399480

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© 2023 Ge et al.

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FTO/IGF2BP2 inhibited NLRP3 inflammasome activation in OGD/R microglia by downregulating the m6A level of NLRP3. (A) The potential m6A sites on NLRP3 mRNA were analyzed by m6Avar. (B and C) RIP assays were used to detect the interaction between m6A/IGF2BP2 and NLRP3 mRNA (n=3). (D and E) The expression of FTO in primary microglia was detected by Western blot assay. β-actin was used as an internal control (n=3). (F and G) RIP assays were used to detect the interaction between m6A/IGF2BP2 and NLRP3 mRNA in primary microglia infected with shRNA-FTO for 48 h (n=3). (H and I) The expression of NLRP3 in primary microglia was detected by Western blot assay. β-actin was used as an internal control (n=3). (J) The content of IL-1β in the supernatant of primary microglia was detected by ELISA assay (n=3). Results were shown as mean ± SD. **P <0.01, ****P <0.0001 versus the IgG group, *P <0.05 versus the shC group; ***P <0.001, ****P <0.0001 versus the IgG group in sh-C; ^###P <0.001, ^####P <0.0001 versus the IgG group in sh-FTO; *P <0.05, ***P <0.001 versus the Control group; ^###P <0.001 versus the OGD/R+sh-C group.